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Allelic differences in Medicago truncatula NIP/LATD mutants correlate with their encoded proteins' transport activities in planta.
Salehin, Mohammad; Huang, Ying-Sheng; Bagchi, Rammyani; Sherrier, D Janine; Dickstein, Rebecca.
Afiliação
  • Salehin M; Department of Biological Sciences; University of North Texas; Denton, TX USA.
  • Huang YS; Department of Biological Sciences; University of North Texas; Denton, TX USA.
  • Bagchi R; Department of Biological Sciences; University of North Texas; Denton, TX USA.
  • Sherrier DJ; Department of Plant and Soil Science; Delaware Biotechnology Institute; Newark, DE USA.
  • Dickstein R; Department of Biological Sciences; University of North Texas; Denton, TX USA.
Plant Signal Behav ; 8(2): e22813, 2013 Feb.
Article em En | MEDLINE | ID: mdl-23154505
Medicago truncatula NIP/LATD gene, required for symbiotic nitrogen fixing nodule and root architecture development, encodes a member of the NRT1(PTR) family that demonstrates high-affinity nitrate transport in Xenopus laevis oocytes. Of three Mtnip/latd mutant proteins, one retains high-affinity nitrate transport in oocytes, while the other two are nitrate-transport defective. To further examine the mutant proteins' transport properties, the missense Mtnip/latd alleles were expressed in Arabidopsis thaliana chl1-5, resistant to the herbicide chlorate because of a deletion spanning the nitrate transporter AtNRT1.1(CHL1) gene. Mtnip-3 expression restored chlorate sensitivity in the Atchl1-5 mutant, similar to wild type MtNIP/LATD, while Mtnip-1 expression did not. The high-affinity nitrate transporter AtNRT2.1 gene was expressed in Mtnip-1 mutant roots; it did not complement, which could be caused by several factors. Together, these findings support the hypothesis that MtNIP/LATD may have another biochemical activity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Medicago truncatula Idioma: En Revista: Plant Signal Behav Ano de publicação: 2013 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Medicago truncatula Idioma: En Revista: Plant Signal Behav Ano de publicação: 2013 Tipo de documento: Article