Mechanism of DNA resection during intrachromosomal recombination and immunoglobulin class switching.
J Exp Med
; 210(1): 115-23, 2013 Jan 14.
Article
em En
| MEDLINE
| ID: mdl-23254285
ABSTRACT
DNA double-strand breaks (DSBs) are byproducts of normal cellular metabolism and obligate intermediates in antigen receptor diversification reactions. These lesions are potentially dangerous because they can lead to deletion of genetic material or chromosome translocation. The chromatin-binding protein 53BP1 and the histone variant H2AX are required for efficient class switch (CSR) and V(D)J recombination in part because they protect DNA ends from resection and thereby favor nonhomologous end joining (NHEJ). Here, we examine the mechanism of DNA end resection in primary B cells. We find that resection depends on both CtBP-interacting protein (CtIP, Rbbp8) and exonuclease 1 (Exo1). Inhibition of CtIP partially rescues the CSR defect in 53BP1- and H2AX-deficient lymphocytes, as does interference with the RecQ helicases Bloom (Blm) and Werner (Wrn). We conclude that CtIP, Exo1, and RecQ helicases contribute to the metabolism of DNA ends during DSB repair in B lymphocytes and that minimizing resection favors efficient CSR.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Recombinação Genética
/
Isotipos de Imunoglobulinas
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Linfócitos B
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Proteínas de Transporte
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Proteínas de Ciclo Celular
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Exodesoxirribonucleases
Limite:
Animals
Idioma:
En
Revista:
J Exp Med
Ano de publicação:
2013
Tipo de documento:
Article