Protective effect of oestradiol in the coeliac ganglion against ovarian apoptotic mechanism on dioestrus.
J Steroid Biochem Mol Biol
; 135: 60-6, 2013 May.
Article
em En
| MEDLINE
| ID: mdl-23313240
ABSTRACT
The aims of this work were to investigate if oestradiol 10(-8)M in the incubation media of either the ovary alone (OV) or the ganglion compartment of an ex vivo coeliac ganglion-superior ovarian nerve-ovary system (a) modifies the release of ovarian progesterone (P4) and oestradiol (E2) on dioestrus II, and (b) modifies the ovarian gene expression of 3ß-HSD and 20α-HSD enzymes and markers of apoptosis. The concentration of ovarian P4 release was measured in both experimental schemes, and ovarian P4 and E2 in the ex vivo system by RIA at different times. The expression of 3ß-hydroxysteroid dehydrogenase, 20α-hydroxysteroid dehydrogenase and antiapoptotic bcl-2 and proapoptotic bax by RT-PCR were determined. E2 added in the coeliac ganglion caused an increase in the ovarian release of the P4, E2 and 3ß-HSD, while in the ovary incubation alone it decreased P4 and 3ß-HSD but increased and 20α-HSD and bax/bcl-2 ratio. It is concluded that through a direct effect on the ovary, E2 promotes luteal regression in DII rats, but the addition of E2 in the coeliac ganglion does not have the same effect. The peripheral nervous system, through the superior ovarian nerve, has a protective effect against the apoptotic mechanism on DII.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ovário
/
Diestro
/
Apoptose
/
Estradiol
/
Gânglios Simpáticos
Limite:
Animals
Idioma:
En
Revista:
J Steroid Biochem Mol Biol
Ano de publicação:
2013
Tipo de documento:
Article