Your browser doesn't support javascript.
loading
Epigenetic marking and repression of porcine endogenous retroviruses.
Wolf, Gernot; Nielsen, Anders Lade; Mikkelsen, Jacob Giehm; Pedersen, Finn Skou.
Afiliação
  • Wolf G; Department of Molecular Biology and Genetics, University of Aarhus, DK-8000 Aarhus C, Denmark.
  • Nielsen AL; Department of Biomedicine, University of Aarhus, DK-8000 Aarhus C, Denmark.
  • Mikkelsen JG; Department of Biomedicine, University of Aarhus, DK-8000 Aarhus C, Denmark.
  • Pedersen FS; Department of Molecular Biology and Genetics, University of Aarhus, DK-8000 Aarhus C, Denmark.
J Gen Virol ; 94(Pt 5): 960-970, 2013 May.
Article em En | MEDLINE | ID: mdl-23324470
Endogenous retroviruses (ERVs) are remnants of retroviral germ line infections and have been identified in all mammals investigated so far. Although the majority of ERVs are degenerated, some mammalian species, such as mice and pigs, carry replication-competent ERVs capable of forming infectious viral particles. In mice, ERVs are silenced by DNA methylation and histone modifications and some exogenous retroviruses were shown to be transcriptionally repressed after integration by a primer-binding site (PBS) targeting mechanism. However, epigenetic repression of porcine ERVs (PERVs) has remained largely unexplored so far. In this study, we screened the pig genome for PERVs using LTRharvest, a tool for de novo detection of ERVs, and investigated various aspects of epigenetic repression of three unrelated PERV families. We found that these PERV families are differentially up- or downregulated upon chemical inhibition of DNA methylation and histone deacetylation in cultured porcine cells. Furthermore, chromatin immunoprecipitation analysis revealed repressive histone methylation marks at PERV loci in primary porcine embryonic germ cells and immortalized embryonic kidney cells. PERV elements belonging to the PERV-γ1 family, which is the only known PERV family that has remained active up to the present, were marked by significantly higher levels of histone methylations than PERV-γ2 and PERV-ß3 proviruses. Finally, we tested three PERV-associated PBS sequences for repression activity in murine and porcine cells using retroviral transduction experiments and showed that none of these PBS sequences induced immediate transcriptional silencing in the tested primary porcine cells.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças dos Suínos / Regulação Viral da Expressão Gênica / Provírus / Retrovirus Endógenos / Repressão Epigenética Limite: Animals Idioma: En Revista: J Gen Virol Ano de publicação: 2013 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças dos Suínos / Regulação Viral da Expressão Gênica / Provírus / Retrovirus Endógenos / Repressão Epigenética Limite: Animals Idioma: En Revista: J Gen Virol Ano de publicação: 2013 Tipo de documento: Article