Mapping O-glycosylation of apolipoprotein C-III in MALDI-FT-ICR protein profiles.
Proteomics
; 13(6): 992-1001, 2013 Mar.
Article
em En
| MEDLINE
| ID: mdl-23335445
ABSTRACT
Ultrahigh resolution MALDI-FT-ICR profiles were obtained from human serum samples that were processed using a fully automated RPC18-based magnetic bead method. Proteins were profiled from m/z value 6630 with a resolving power of 73 000 up to m/z value 12 600 with a resolving power of 37 000. In this study, a detailed evaluation was performed of the isoforms of apolipoprotein C-III, i.e. the different mucin-type core 1 O-glycans with the addition of one or two sialic acid residues. The MALDI-FT-ICR profiles are discussed with regard to reproducibility of the signal intensities as well as the accurate mass measurements. ESI-FT-ICR-MS/MS analyses of the same serum samples were performed to confirm the identity of apolipoprotein C-III glycoforms.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Mapeamento de Peptídeos
/
Processamento de Proteína Pós-Traducional
/
Apolipoproteína C-III
Limite:
Humans
Idioma:
En
Revista:
Proteomics
Ano de publicação:
2013
Tipo de documento:
Article