The small molecule dispergo tubulates the endoplasmic reticulum and inhibits export.
Mol Biol Cell
; 24(7): 1020-9, 2013 Apr.
Article
em En
| MEDLINE
| ID: mdl-23389632
The mammalian endoplasmic reticulum (ER) is an organelle that maintains a complex, compartmentalized organization of interconnected cisternae and tubules while supporting a continuous flow of newly synthesized proteins and lipids to the Golgi apparatus. Using a phenotypic screen, we identify a small molecule, dispergo, that induces reversible loss of the ER cisternae and extensive ER tubulation, including formation of ER patches comprising densely packed tubules. Dispergo also prevents export from the ER to the Golgi apparatus, and this traffic block results in breakdown of the Golgi apparatus, primarily due to maintenance of the constitutive retrograde transport of its components to the ER. The effects of dispergo are reversible, since its removal allows recovery of the ER cisternae at the expense of the densely packed tubular ER patches. This recovery occurs together with reactivation of ER-to-Golgi traffic and regeneration of a functional Golgi with correct morphology. Because dispergo is the first small molecule that reversibly tubulates the ER and inhibits its export function, it will be useful in studying these complex processes.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Compostos Orgânicos
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Retículo Endoplasmático
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Exocitose
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Complexo de Golgi
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Compostos Heterocíclicos de 4 ou mais Anéis
Limite:
Animals
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Humans
Idioma:
En
Revista:
Mol Biol Cell
Ano de publicação:
2013
Tipo de documento:
Article