Quantifying loss of CD34+ cells collected by apheresis after processing for freezing and post-thaw.
Transfus Apher Sci
; 48(2): 241-6, 2013 Apr.
Article
em En
| MEDLINE
| ID: mdl-23394728
ABSTRACT
INTRODUCTION:
CD34(+) cells collected for autologous bone marrow transplantation (BMT) are usually quantified in the apheresis product after collection, but the necessity to repeat these measures post-thaw is controversial.METHODS:
We examined the loss of CD34(+) cells after collection, preparation for freezing and post-thaw in apheresis products collected for BMT.RESULTS:
Median number of CD34(+) cells collected per unit was 1.61×10(6)/kg, viability 97-100%. This number decreased to 1.38×10(6)/kg, viability 96-100% before freezing and was 1.17×10(6)/kg post-thaw. Viability decreased to 86-98%. The relative loss of viable PBHPC showed an inverse correlation with the ratio "CD34(+) cells/total nucleated cells" (r=-0.45; p=<0.0005). This relative loss was largest in patients with Hodgkin's lymphoma.CONCLUSION:
Cryopreservation and thawing of PBHPCs in leukapheresis products provokes a small but significant stem cell loss. So, quantification of viable CD34(+) cells post-thaw is important, especially in poorly mobilizing patients. Besides, the ratio "CD34(+) cells/total nucleated cells" after leukapheresis is an important parameter for prediction of neutrophil recovery after BMT.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfoma não Hodgkin
/
Células da Medula Óssea
/
Criopreservação
/
Transplante de Medula Óssea
/
Leucaférese
/
Antígenos CD34
/
Mieloma Múltiplo
Tipo de estudo:
Prognostic_studies
Limite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
Transfus Apher Sci
Ano de publicação:
2013
Tipo de documento:
Article