Grape seed extracts inhibit platelet aggregation by inhibiting protein tyrosine phosphatase.
Clin Appl Thromb Hemost
; 20(3): 278-84, 2014 Apr.
Article
em En
| MEDLINE
| ID: mdl-23478570
ABSTRACT
Platelets play an important role in various thrombotic diseases, including myocardial infarction. Because red wine consumption is inversely associated with death due to ischemic heart diseases, the effects of grape components on platelet function have been extensively investigated. Grape seed extracts (GSEs) reportedly inhibit platelet aggregation; however, the underlying mechanism has not been elucidated. We discovered that GSEs inhibit platelet aggregation induced by collagen and thrombin-receptor agonist peptide and increase basal levels of tyrosine phosphorylation, which was also observed in the presence of a protein tyrosine phosphatase (PTP) inhibitor. An in vitro phosphatase assay indicated that GSE dose dependently inhibited PTP-1B and Src homology 2 domain-containing phosphatase-1 activity, which positively regulates platelet aggregation. We propose that GSEs inhibit platelet aggregation by inhibiting tyrosine phosphatase activity. Moreover, we showed that GSE ingestion inhibited platelet aggregation in mice without enhancing tail bleeding, implying that GSE supplementation might be beneficial to prevention of thrombotic diseases.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plaquetas
/
Ativação Plaquetária
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Agregação Plaquetária
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Proteínas Tirosina Fosfatases
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Inibidores Enzimáticos
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Extrato de Sementes de Uva
Limite:
Animals
Idioma:
En
Revista:
Clin Appl Thromb Hemost
Ano de publicação:
2014
Tipo de documento:
Article