Regulatory T cells, interleukin (IL)-6, IL-8, vascular endothelial growth factor (VEGF), CXCL10, CXCL11, epidermal growth factor (EGF) and hepatocyte growth factor (HGF) as surrogate markers of host immunity in patients with renal cell carcinoma.
BJU Int
; 112(5): 686-96, 2013 Sep.
Article
em En
| MEDLINE
| ID: mdl-23495770
ABSTRACT
OBJECTIVE:
To identify a phenotype that could be informative and prognostic in patients with renal cell carcinoma (RCC) peripheral blood was evaluated for TH1, TH2, regulatory T cells (Tregs), natural killer (NK) and NKT cells and for cytokines/chemokines. PATIENTS ANDMETHODS:
Peripheral blood from 77 patients with RCC and 40 healthy controls was evaluated by flow cytometry using monoclonal antibodies against CD4, CD25, FoxP3, CD45RA, CD45RO, CD152, CD184, CD279, CD3, CD16, CD56, CD161, CD158a, CD4, CD26, CD30, CD183 and CD184. A concomitant evaluation of 38 molecules was conducted in patients' serum using a multiplex biometric ELISA-based immunoassay.RESULTS:
The number of NK cells CD3â»/CD16âº, CD3â»/CD16âº/CD161⺠(NK) and CD3â»/CD16âº/CD161âº/CD158a⺠(NK- Kir 2+) was greater in the patients with RCC (P < 0.05); and the number of Treg cells CD4âº/CD25(high+)/FOXP3⺠and the subset CD4âº/CD25(high+)/FOXP3âº/CD45RA⺠(naïve) and CD45R0âº(memory) cells, were greater in the patients with RCC (P < 0.001). An increase in the following was observed in the serum of patients with RCC compared with healthy controls interleukin (IL)-4, IL-6, IL-8, IL-10, G-CSF, CXCL10, CXCL11, hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF). According to Ingenuity Pathway Analysis (IPA), CXCL10, IL-6, IL-8, epidermal growth factor (EGF), HGF and VEGF were associated with a network that controls cellular movement, tissue development and cellular growth. Kaplan-Meier analysis for disease-free survival showed that high numbers of CD4âº/CD25(high+)/FOXP3âº/CD45RA⺠(Treg naïve) and low numbers of CD3â»/CD16âº/CD161âº/CD158a⺠(NK-Kir+) cells predict short disease-free survival in patients with RCC.CONCLUSION:
Concomitant evaluation of Treg (CD4âº/CD25(high+)/FOXP3⺠and CD4âº/CD25(high+)/FOXP3âº/CD45RAâº) and of six soluble factors (IL-6, IL-8 ,VEGF, CXCL10, CXCL11, EGF, HGF) might be a surrogate marker of host immunity in patients with RCC.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Matadoras Naturais
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Carcinoma de Células Renais
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Linfócitos T Reguladores
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Neoplasias Renais
Tipo de estudo:
Prognostic_studies
Limite:
Adult
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Aged
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Aged80
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
BJU Int
Ano de publicação:
2013
Tipo de documento:
Article