Species-specific multiplex PCR for the diagnosis of Brucella ovis, Actinobacillus seminis, and Histophilus somni infection in rams.
BMC Vet Res
; 9: 51, 2013 Mar 21.
Article
em En
| MEDLINE
| ID: mdl-23514236
ABSTRACT
BACKGROUND:
Infectious ovine epididymitis results in substantial economic losses worldwide due to reproductive failure and culling of breeders. The most common causative agents of these infections are Brucella ovis, Actinobacillus seminis, and Histophilus somni. The aim of this study was to develop a multiplex PCR assay for simultaneous detection of Brucella ovis, Actinobacillus seminis, and Histophilus somni with species-specific primers applied to biological samples for molecular diagnosis of these infections.RESULTS:
The multiplex assay was capable of detecting B. ovis, A. seminis, and H. somni DNA simultaneously from genomic bacterial DNA samples and pool of semen samples from experimentally infected rams. The method was highly specific since it did not amplify DNA from other bacterial species that can potentially cause epididymitis in rams as well as species phylogenetically related to B. ovis. All negative control samples were negative in PCR multiplex assay. Urine can be used as an alternative to semen samples.CONCLUSIONS:
The species-specific multiplex PCR assay developed in this study can be successfully used for the detection of three of the most common bacterial causes of ovine epididymitis.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
/
3_ND
Base de dados:
MEDLINE
Assunto principal:
Doenças dos Ovinos
/
Actinobacilose
/
Brucelose
/
Pasteurellaceae
/
Actinobacillus
/
Infecções por Pasteurellaceae
/
Brucella ovis
/
Reação em Cadeia da Polimerase Multiplex
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
BMC Vet Res
Ano de publicação:
2013
Tipo de documento:
Article