Strategies for production of active eukaryotic proteins in bacterial expression system.
Asian Pac J Trop Biomed
; 2(2): 159-62, 2012 Feb.
Article
em En
| MEDLINE
| ID: mdl-23569889
ABSTRACT
Bacteria have long been the favorite expression system for recombinant protein production. However, the flaw of the system is that insoluble and inactive proteins are co-produced due to codon bias, protein folding, phosphorylation, glycosylation, mRNA stability and promoter strength. Factors are cited and the methods to convert to soluble and active proteins are described, for example a tight control of Escherichia coli milieu, refolding from inclusion body and through fusion technology.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes
/
Clonagem Molecular
/
Escherichia coli
Idioma:
En
Revista:
Asian Pac J Trop Biomed
Ano de publicação:
2012
Tipo de documento:
Article