Effect of human mesenchymal stem cells on the growth of HepG2 and Hela cells.
Cell Struct Funct
; 38(1): 109-21, 2013.
Article
em En
| MEDLINE
| ID: mdl-23575155
ABSTRACT
Human mesenchymal stem cells (hMSCs) accumulate at carcinomas and have a great impact on cancer cell's behavior. Here we demonstrated that hMSCs could display both the promotional and inhibitive effects on growth of HepG2 and Hela cells by using the conditioned media, indirect co-culture, and cell-to-cell co-culture. Cell growth was increased following the addition of lower proportion of hMSCs while decreased by treatment of higher proportion of hMSCs. We also established a novel noninvasive label way by using internalizing quantum dots (i-QDs) for study of cell-cell contact in the co-culture, which was effective and sensitive for both tracking and distinguishing different cells population without the disturbance of cells. Furthermore, we investigated the role of hMSCs in regulation of cell growth and showed that mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) signaling pathways were involved in hMSC-mediated cell inhibition and proliferation. Our findings suggested that hMSCs regulated cancer cell function by providing a suitable environment, and the discovery from the study would provide some clues for development of effective strategy for hMSC-based cancer therapies.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células HeLa
/
Comunicação Celular
/
Proliferação de Células
/
Células Hep G2
/
Células-Tronco Mesenquimais
Limite:
Female
/
Humans
Idioma:
En
Revista:
Cell Struct Funct
Ano de publicação:
2013
Tipo de documento:
Article