One-pot three-enzyme synthesis of UDP-Glc, UDP-Gal, and their derivatives.
Carbohydr Res
; 373: 76-81, 2013 May 24.
Article
em En
| MEDLINE
| ID: mdl-23584237
ABSTRACT
A UTP-glucose-1-phosphate uridylyltransferase (SpGalU) and a galactokinase (SpGalK) were cloned from Streptococcus pneumoniae TIGR4 and were successfully used to synthesize UDP-galactose (UDP-Gal), UDP-glucose (UDP-Glc), and their derivatives in an efficient one-pot reaction system. The reaction conditions for the one-pot multi-enzyme synthesis were optimized and nine UDP-Glc/Gal derivatives were synthesized. Using this system, six unnatural UDP-Gal derivatives, including UDP-2-deoxy-Galactose and UDP-GalN3 which were not accepted by other approach, can be synthesized efficiently in a one pot fashion. More interestingly, this is the first time it has been reported that UDP-Glc can be synthesized in a simpler one-pot three-enzyme synthesis reaction system.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Uridina Difosfato Galactose
/
Uridina Difosfato Glucose
/
Galactoquinase
/
UTP-Glucose-1-Fosfato Uridililtransferase
Idioma:
En
Revista:
Carbohydr Res
Ano de publicação:
2013
Tipo de documento:
Article