Ground state structure of D75N mutant of sensory rhodopsin II in complex with its cognate transducer.
J Photochem Photobiol B
; 123: 55-8, 2013 Jun 05.
Article
em En
| MEDLINE
| ID: mdl-23619282
ABSTRACT
The complex of sensory rhodopsin II (NpSRII) with its cognate transducer (NpHtrII) mediates negative phototaxis in halobacteria Natronomonas pharaonis. Upon light activation NpSRII triggers, by means of NpHtrII, a signal transduction chain homologous to the two component system in eubacterial chemotaxis. Here we report on the crystal structure of the ground state of the mutant NpSRII-D75N/NpHtrII complex in the space group I212121. Mutations of this aspartic acid in light-driven proton pumps dramatically modify or/and inhibit protein functions. However, in vivo studies show that the similar D75N mutation retains functionality of the NpSRII/NpHtrII complex. The structure provides the molecular basis for the explanation of the unexpected observation that the wild and the mutant complexes display identical physiological response on light excitation.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carotenoides
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Proteínas Arqueais
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Halorrodopsinas
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Rodopsinas Microbianas
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Rodopsinas Sensoriais
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Peptídeos e Proteínas de Sinalização Intracelular
Idioma:
En
Revista:
J Photochem Photobiol B
Ano de publicação:
2013
Tipo de documento:
Article