Protection against experimental melioidosis following immunization with live Burkholderia thailandensis expressing a manno-heptose capsule.
Clin Vaccine Immunol
; 20(7): 1041-7, 2013 Jul.
Article
em En
| MEDLINE
| ID: mdl-23677322
ABSTRACT
Melioidosis is a severe infectious disease caused by Burkholderia pseudomallei. It is highly resistant to antibiotic treatment, and there is currently no licensed vaccine. Burkholderia thailandensis is a close relative of Burkholderia pseudomallei but is essentially avirulent in mammals. In this report, we detail the protective efficacy of immunization with live B. thailandensis E555, a strain which has been shown to express an antigenic capsule similar to that of B. pseudomallei. Immunization with E555 induced significant protection against a lethal intraperitoneal B. pseudomallei challenge in a mouse model of infection, with no mice succumbing to infection over the course of the study, even with challenges of up to 6,000 median lethal doses. By comparison, mice immunized with B. thailandensis not expressing a B. pseudomallei-like capsule had significantly decreased levels of protection. E555-immunized mice had significantly higher levels of IgG than mice immunized with noncapsulated B. thailandensis, and these antibody responses were primarily directed against the capsule.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
4_TD
Base de dados:
MEDLINE
Assunto principal:
Vacinas Bacterianas
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Burkholderia
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Melioidose
Limite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Clin Vaccine Immunol
Ano de publicação:
2013
Tipo de documento:
Article