An efficient and non-enzymatic method for isolation and culture of endothelial cells from the nidus of human cerebral arteriovenous malformations.
Neurosci Lett
; 548: 21-6, 2013 Aug 26.
Article
em En
| MEDLINE
| ID: mdl-23748040
ABSTRACT
In this report, we describe an efficient and non-enzymatic method for isolating and culturing endothelial cells (ECs) from the nidus of surgically resected arteriovenous malformation (AVM) specimens. These cultured cells possessed typical phenotypic markers (i.e. von Willebrand factor and CD34), as well as morphological and ultrastructural characteristics of ECs. However, they had activated Notch-1 signaling, which plays a critical role in the development of AVM. The present study suggests that hypoxic endothelial cells from the nidus of human cerebral arteriovenous malformation (CAVMECs) have angiogenic potentials, as our data showed that VEGF gene expression and cell proliferation were more evident with prolonged hypoxia. In our study, we successfully used the vascular tissue explants adherent method to isolate and culture CAVMECs with high purity. This may prove to be a useful tool for studying the molecular mechanisms that mediate abnormal vessel development and maintenance in AVM.
Palavras-chave
AVM; Arteriovenous malformation; CAVM; CAVMECs; ECs; Endothelial cell; GFAP; Isolation method; NICD; PBS; arteriovenous malformation; cerebral arteriovenous malformation; endothelial cells; endothelial cells from the nidus of human cerebral arteriovenous malformation; glial fibrillary acidic protein; intracellular domain of Notch.; phosphate buffered saline; vWF; von Willebrand factor; α-SMA; α-smooth muscle actin
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Manejo de Espécimes
/
Encéfalo
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Malformações Arteriovenosas Intracranianas
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Técnicas de Cultura de Células
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Células Endoteliais
Limite:
Adolescent
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Adult
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Female
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Humans
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Male
Idioma:
En
Revista:
Neurosci Lett
Ano de publicação:
2013
Tipo de documento:
Article