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ECM modulated early kidney development in embryonic organ culture.
Sebinger, David D R; Ofenbauer, Andreas; Gruber, Petra; Malik, Susann; Werner, Carsten.
Afiliação
  • Sebinger DD; Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, 01069 Dresden, Germany.
Biomaterials ; 34(28): 6670-82, 2013 Sep.
Article em En | MEDLINE | ID: mdl-23773818
The use of exogenous signals is gaining importance in renal regenerative therapies. We wanted to explore the role of extracellular matrix (ECM) constituents on renal structure formation during renal organogenesis. We used a recently established organ culture setup to expose embryonic kidney rudiments directly to a large set of surface-immobilized or dissolved ECM molecules and growth factors. Organ culture was also performed on immobilized adult kidney ECM extracts and on reactive polymer films without any biomolecular components. The applied conditions resulted in distinct differences of organ phenotypes, underlining the multifaceted role of exogenous signals during kidney development. Specific ECM components, including collagen I and laminin, supported nephronal and tubular structure formation of the developing organ. ECM biopolymers, e.g. hyaluronic acid, were found to determine the fate of developing explants in a concentration- and molecular weight-dependent manner. The organ culture system used was an effective and robust means to identify exogenous signals that direct kidney development. This system can provide valuable insight for future regenerative therapies of kidney diseases.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas de Cultura de Órgãos / Engenharia Tecidual / Matriz Extracelular / Rim Tipo de estudo: Prognostic_studies Limite: Animals / Pregnancy Idioma: En Revista: Biomaterials Ano de publicação: 2013 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas de Cultura de Órgãos / Engenharia Tecidual / Matriz Extracelular / Rim Tipo de estudo: Prognostic_studies Limite: Animals / Pregnancy Idioma: En Revista: Biomaterials Ano de publicação: 2013 Tipo de documento: Article