Your browser doesn't support javascript.
loading
Basophils help establish protective immunity induced by irradiated larval vaccination for filariasis.
Torrero, Marina N; Morris, C Paul; Mitre, Blima K; Hübner, Marc P; Fox, Ellen M; Karasuyama, Hajime; Mitre, Edward.
Afiliação
  • Torrero MN; Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
Vaccine ; 31(36): 3675-82, 2013 Aug 12.
Article em En | MEDLINE | ID: mdl-23777951
ABSTRACT
Basophils are increasingly recognized as playing important roles in the immune response toward helminths. In this study, we evaluated the role of basophils in vaccine-mediated protection against filariae, tissue-invasive parasitic nematodes responsible for diseases such as elephantiasis and river blindness. Protective immunity and immunological responses were assessed in BALB/c mice vaccinated with irradiated L3 stage larvae and depleted of basophils with weekly injections of anti-CD200R3 antibody. Depletion of basophils after administration of the vaccination regimen but before challenge infection did not alter protective immunity. In contrast, basophil depletion initiated prior to vaccination and continued after challenge infection significantly attenuated the protective effect conferred by vaccination. Vaccine-induced cellular immune responses to parasite antigen were substantially decreased in basophil-depleted mice, with significant decreases in CD4(+) T-cell production of IL-4, IL-5, IL-10, and IFN-γ. Interestingly, skin mast cell numbers, which increased significantly after vaccination with irradiated L3 larvae, were unchanged after vaccination in basophil-depleted mice. These findings demonstrate that basophils help establish the immune responses responsible for irradiated L3 vaccine protection.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Basófilos / Vacinas / Filariose / Filarioidea Limite: Animals Idioma: En Revista: Vaccine Ano de publicação: 2013 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Basófilos / Vacinas / Filariose / Filarioidea Limite: Animals Idioma: En Revista: Vaccine Ano de publicação: 2013 Tipo de documento: Article