Molecular characterization and functional analysis of Toll-like receptor 3 gene in orange-spotted grouper (Epinephelus coioides).
Gene
; 527(1): 174-82, 2013 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-23792060
ABSTRACT
Toll-like receptor 3 (TLR3) plays an important role in activating innate immune responses during viral infection. In this report, TLR3 (EcTLR3) was characterized and analyzed for the first time in Epinephelus coioides. The full-length EcTLR3 cDNA is predicted to encode a 909 amino acid polypeptide that contains a signal peptide sequence, 18 leucine-rich repeat (LRR) motifs, a transmembrane region and a Toll/interleukin-1 receptor (TIR) domain. Quantitative real-time PCR revealed that the EcTLR3 mRNA was much more abundant in the liver than in other immune organs, and that the expression levels were very low in hemocyte and muscle. During development of the grouper, the levels of EcTLR3 transcripts increased with age, with very low expression levels at the early stages of development. EcTLR3 mRNA levels were examined in the liver at different times after treatment with polyriboinosinic polyribocytidylic acid (Poly IC), and in nervous necrosis virus (NNV)-infected larval groupers. The results suggested that EcTLR3 plays an important role in a fish's defense against viral infection.
Palavras-chave
Epinephelus coioides; Gene expression; IHNV; Innate immunity; JNK; Jun N-terminal kinase; LRR; NNV; PAMPs; PRRs; Poly I:C; RNA virus; TIR; TLR3; TLRs; Toll-like receptors; Toll/interleukin-1 receptor; VNN; infectious hematopoietic necrosis virus; leucine-rich repeat; nervous necrosis virus; pathogen-associated molecular patterns; pattern recognition receptors; polyriboinosinic polyribocytidylic acid; viral nervous necrosis
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por Vírus de RNA
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Perciformes
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Nodaviridae
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Proteínas de Peixes
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Receptor 3 Toll-Like
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Doenças dos Peixes
Limite:
Animals
Idioma:
En
Revista:
Gene
Ano de publicação:
2013
Tipo de documento:
Article