[The experience of applying techniques of molecular genetics in identification of clinical strains Pseudomonas aeruginosa].

ABSTRACT

The article presents comparative analysis of application of common bacteriologic and molecular techniques to identify P. aerugenosa. The genetic detection was applied using polymerase chain reaction with genus-specific and species-specific primers and amplification of conservative sites of gens 16S pRNA with successive identification of bacteria by sequenation. It is established that 95% (151) of strains correspond to species of P. aerugenosa detected in primary bacteriologic laboratories and only 8 strains were not blue pus bacillus. Most of strains were closely congenial species 2 isolates belonged to Pseudomonas aeruginosa group, 3 isolates to Pseudomonas putida group, 2 strains to Comamonas species and 1 isolate to Stenotrophomonas maltophilia species. The effectiveness of cultural technique of laboratory diagnostic was demonstrated concerning infections conditioned by P. aerugenosa. This conclusion does not eliminate application of molecular genetic trechnologies in complicated arbitral cases of bacteriologic analysis during monitoring of nosocomial infections.