Spontaneous activation of [FeFe]-hydrogenases by an inorganic [2Fe] active site mimic.
Nat Chem Biol
; 9(10): 607-609, 2013 Oct.
Article
em En
| MEDLINE
| ID: mdl-23934246
Hydrogenases catalyze the formation of hydrogen. The cofactor ('H-cluster') of [FeFe]-hydrogenases consists of a [4Fe-4S] cluster bridged to a unique [2Fe] subcluster whose biosynthesis in vivo requires hydrogenase-specific maturases. Here we show that a chemical mimic of the [2Fe] subcluster can reconstitute apo-hydrogenase to full activity, independent of helper proteins. The assembled H-cluster is virtually indistinguishable from the native cofactor. This procedure will be a powerful tool for developing new artificial H2-producing catalysts.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Coenzimas
/
Hidrogênio
/
Hidrogenase
/
Ferro
/
Proteínas Ferro-Enxofre
Idioma:
En
Revista:
Nat Chem Biol
Ano de publicação:
2013
Tipo de documento:
Article