Dereplication of bioactive constituents of the genus hypericum using LC-(+,-)-ESI-MS and LC-PDA techniques: Hypericum triquterifolium as a case study.
Saudi Pharm J
; 17(4): 269-74, 2009 Oct.
Article
em En
| MEDLINE
| ID: mdl-23960710
ABSTRACT
Utilizing liquid chromatography-electro spray ionization-mass spectrometry (LC-(+,-)-ESI-MS) and liquid chromatography-photodiode array detection (LC-PDA) techniques, a dereplication strategy for the analysis of the secondary metabolites constituents of the genus Hypericum has been developed. From the crude methanolic extract of the aerial parts of H. triquetrifolium (leaves, stems, and flowers) and on the basis of their UV-profiles, chromatographic retention times and (+,-)-ESI-MS (TIC and SIM) mass spectral data, seven known (1-7) compounds were dereplicated fairly rapidly. The compounds were classified into three structural classes phloroglucinols hyperfirin and adhyperfirin; naphthodianthrones hypericin, pseudo-hypericin, proto-hypericin, and protopseudo-hypericin; and the flavonoid rutin.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Saudi Pharm J
Ano de publicação:
2009
Tipo de documento:
Article