Global profiling of stimulus-induced polyadenylation in cells using a poly(A) trap.
Nat Chem Biol
; 9(11): 671-3, 2013 Nov.
Article
em En
| MEDLINE
| ID: mdl-23995769
ABSTRACT
Polyadenylation of mRNA leads to increased protein expression in response to diverse stimuli, but it is difficult to identify mRNAs that become polyadenylated in living cells. Here we describe a click chemistry-compatible nucleoside analog that is selectively incorporated into poly(A) tails of transcripts in cells. Next-generation sequencing of labeled mRNAs enables a transcriptome-wide profile of polyadenylation and provides insights into the mRNA sequence elements that are correlated with polyadenylation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poli A
/
Poliadenilação
Idioma:
En
Revista:
Nat Chem Biol
Ano de publicação:
2013
Tipo de documento:
Article