Fast spectral coherent anti-Stokes Raman scattering microscopy with high-speed tunable picosecond laser.
J Biomed Opt
; 18(9): 096009, 2013 Sep.
Article
em En
| MEDLINE
| ID: mdl-24013358
ABSTRACT
We develop a coherent anti-Stokes Raman scattering (CARS) microscopy system equipped with a tunable picosecond laser for high-speed wavelength scanning. An acousto-optic tunable filter (AOTF) is integrated in the laser cavity to enable wavelength scanning by varying the radio frequency waves applied to the AOTF crystal. An end mirror attached on a piezoelectric actuator and a pair of parallel plates driven by galvanometer motors are also introduced into the cavity to compensate for changes in the cavity length during wavelength scanning to allow synchronization with another picosecond laser. We demonstrate fast spectral imaging of 3T3-L1 adipocytes every 5 cm-1 in the Raman spectral region around 2850 cm-1 with an image acquisition time of 120 ms. We also demonstrate fast switching of Raman shifts between 2100 and 2850 cm-1, corresponding to CD2 symmetric stretching and CH2 symmetric stretching vibrations, respectively. The fast-switching CARS images reveal different locations of recrystallized deuterated and nondeuterated stearic acid.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Análise Espectral Raman
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Processamento de Imagem Assistida por Computador
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Microscopia
Limite:
Animals
Idioma:
En
Revista:
J Biomed Opt
Ano de publicação:
2013
Tipo de documento:
Article