[Paraquat involves differentiation of human neural stem cells via Notch signaling].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
; 31(7): 492-5, 2013 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-24053911
ABSTRACT
OBJECTIVE:
To investigate effects of paraquat on the mRNA expression of key elements of Notch signaling (Notch1, Jagged1 and DTX1) during differentiation process of human neural stem cells (hNSCs).METHODS:
hNSCs exposed to PQ at the concentrations 0.10, 1.00, 10.00 M. Cell proliferation ability was assessed using MTT assay and mRNA expressions of Notch1, Jagged1 and DTX1 were detected by Real-time RT-PCR at 2, 4, 8, 12 d of differentiation.RESULTS:
Compared with control group, NOTCH1, JAG1 mRNA expression levels exposed to PQ at the concentration of 0.10 M significantly reduced at 2, 4, 8 d and significantly went up at 12d (P < 0.01). Compared with control group, NOTCH1, JAG1 and DTX1 mRNA expression levels exposed to PQ at the concentration of 10.00 M significantly reduced at 2, 8, 12 d (P < 0.01). PQ could down-regulate Notch1, Jagged1 and DTX1 mRNA expressions at the early stage of differentiation, then up-regulate Notch1 mRNA expression, and down-regulate Notch1, Jagged1 and DTX1 mRNA expressions at the end of differentiation.CONCLUSION:
Notch signaling pathway may be involved in differentiation of neural stem cell exposed to PQ.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Paraquat
/
Proteínas de Ligação ao Cálcio
/
Peptídeos e Proteínas de Sinalização Intercelular
/
Ubiquitina-Proteína Ligases
/
Receptor Notch1
/
Células-Tronco Neurais
/
Proteínas de Membrana
Limite:
Humans
Idioma:
Zh
Revista:
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
Ano de publicação:
2013
Tipo de documento:
Article