Smart-seq2 for sensitive full-length transcriptome profiling in single cells.
Nat Methods
; 10(11): 1096-8, 2013 Nov.
Article
em En
| MEDLINE
| ID: mdl-24056875
ABSTRACT
Single-cell gene expression analyses hold promise for characterizing cellular heterogeneity, but current methods compromise on either the coverage, the sensitivity or the throughput. Here, we introduce Smart-seq2 with improved reverse transcription, template switching and preamplification to increase both yield and length of cDNA libraries generated from individual cells. Smart-seq2 transcriptome libraries have improved detection, coverage, bias and accuracy compared to Smart-seq libraries and are generated with off-the-shelf reagents at lower cost.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Perfilação da Expressão Gênica
/
Análise de Célula Única
/
Transcriptoma
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Methods
Ano de publicação:
2013
Tipo de documento:
Article