[Effects of different vegetation restoration patterns on the diversity of soil nitrogen-fixing microbes in Hulunbeier sandy land, Inner Mongolia of North China].
Ying Yong Sheng Tai Xue Bao
; 24(6): 1639-46, 2013 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-24066552
ABSTRACT
By using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and sequence analysis, this paper studied the nifH gene diversity and community structure of soil nitrogen-fixing microbes in Hulunbeier sandy land of Inner Mongolia under four years management of five vegetation restoration modes, i. e., mixed-planting of Agropyron cristatum, Hedysarum fruticosum, Caragana korshinskii, and Elymus nutans (ACHE) and of Agropyron cristatum and Hedysarum fruticosum (AC), and mono-planting of Caragana korshinskii (UC), Agropyron cristatum (UA), and Hedysarum fruticosum (UH), taking the bare land as the control (CK). There existed significant differences in the community composition of nitrogen-fixing microbes among the five vegetation restoration patterns. The Shannon index of the nifH gene was the highest under ACHE, followed by under AC, UC, UA, and UH, and the lowest in CK. Except that UH and CK had less difference in the Shannon index, the other four vegetation restoration modes had a significantly higher Shannon index than CK (P < 0.05). The phylogenetic analysis showed that the soil nitrogen-fixing microbes under UA, UH, and UC were mainly of cyanobacteria, but the soil nitrogen-fixing microbes under AC and ACHE changed obviously, mainly of proteobacteria, and also of cyanobacteria. The canonical correlation analysis showed that the soil total phosphorus, available phosphorus, total nitrogen, and nitrate nitrogen contents under the five vegetation restoration modes had significant effects on the nitrogen-fixing microbial communities, and there existed significant correlations among the soil total phosphorus, available phosphorus, total nitrogen, and nitrate nitrogen. It was suggested that the variations of the community composition of soil nitrogen-fixing microbes under the five vegetation restoration modes were resulted from the interactive and combined effects of the soil physical and chemical factors.
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Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
Base de dados:
MEDLINE
Assunto principal:
Microbiologia do Solo
/
Bactérias
/
Desenvolvimento Vegetal
/
Nitrogênio
/
Fixação de Nitrogênio
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Ying Yong Sheng Tai Xue Bao
Ano de publicação:
2013
Tipo de documento:
Article