Imaging of neutral lipids and neutral lipid associated proteins.
Methods Cell Biol
; 116: 213-26, 2013.
Article
em En
| MEDLINE
| ID: mdl-24099295
ABSTRACT
Intracellular fat droplets are large and have a distinct morphology, which makes their imaging at the light level simple and informative. We detail how to image the fat droplet core by metabolic labeling with fluorescent fatty acids or lipophilic fluorochromes. Further, we describe the use of indirect immunostaining to image fat droplet proteins and fat cores in the same field. We also address the use of appropriate controls for determining signal specificity and other practical considerations for optimizing image quality.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Corpos de Inclusão
/
Metabolismo dos Lipídeos
/
Ácidos Graxos
/
Proteínas de Membrana
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Revista:
Methods Cell Biol
Ano de publicação:
2013
Tipo de documento:
Article