Mechanisms for adherence of eosinophils to an antibody-coated surface.
Scand J Haematol
; 35(1): 88-95, 1985 Jul.
Article
em En
| MEDLINE
| ID: mdl-2413524
ABSTRACT
Eosinophils may act by degranulation after attachment to a surface. As the mechanisms of adherence are not understood, we have investigated the dependency on Fc(IgG) receptors and other mechanisms by studying the adherence of human eosinophils to albumin-Sepharose beads coated with either specific rabbit IgG antibody, F(ab')2 antibody fragments or serum under different conditions. Adherence to Sephadex beads and albumin-coated microtitration plates was also investigated. 50% of the eosinophils adhered spontaneously to all 3 different surfaces not coated with the antibody, whereas only 25% of neutrophils and less than 10% of mononuclear cells adhered. A small but significant increase in adherence to albumin-Sepharose or albumin-coated plastic occurred after addition of the IgG-antibody, but not after addition of F(ab')2 fragments, indicating that the Fc region was responsible for some increase in adherence. Incubation of eosinophils with IgG-Fc fragments prevented the additional antibody-mediated adherence. As Fc receptor-negative eosinophils adhered almost as well as Fc receptor-positive cells, it appears that the Fc receptors are of minor importance and instead, a nonspecific adherence mechanism, possibly unique for the eosinophil, seems to be the most important in eosinophil adherence to antibody-coated surfaces.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Formação de Roseta
/
Imunoglobulina G
/
Eosinófilos
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Scand J Haematol
Ano de publicação:
1985
Tipo de documento:
Article