Quantification of miRNA by poly(A)-RT-qPCR arrays and verification of target sites in HIV-1 using a one-LTR infectious molecular clone.
Methods Mol Biol
; 1087: 285-96, 2014.
Article
em En
| MEDLINE
| ID: mdl-24158831
ABSTRACT
Quantitative PCR (qPCR) provides a robust method for quantifying DNA species. By combining modern qPCR techniques with the isolation of small RNA, the polyadenylation of the RNA, and the use of reverse transcriptase to create miRNA derived cDNA, it is now possible to use qPCR to quantify miRNA. This method is scalable and provides a useful addition to the retrovirologists' toolbox. Here, we also describe the use of one-LTR infectious molecular clones to verify miRNA target sites within the retroviral LTR.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poli A
/
HIV-1
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Sequências Repetidas Terminais
/
MicroRNAs
Limite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2014
Tipo de documento:
Article