Measuring of Mycobacterium tuberculosis growth. A correlation of the optical measurements with colony forming units.

Peñuelas-Urquides, Katia; Villarreal-Treviño, Licet; Silva-Ramírez, Beatriz; Rivadeneyra-Espinoza, Liliana; Said-Fernández, Salvador; de León, Mario Bermúdez

Peñuelas-Urquides, Katia; Villarreal-Treviño, Licet; Silva-Ramírez, Beatriz; Rivadeneyra-Espinoza, Liliana; Said-Fernández, Salvador; de León, Mario Bermúdez.

Afiliação

Peñuelas-Urquides K; Laboratorio de Biología Molecular, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Monterrey, NL, México. ; Posgrado en Microbiología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, NL, México.

Braz J Microbiol ; 44(1): 287-9, 2013.

Article em En | MEDLINE | ID: mdl-24159318

ABSTRACT

ABSTRACT

The quantification of colony forming units (cfu), turbidity, and optical density at 600 nm (OD600) measurements were used to evaluate Mycobacterium tuberculosis growth. Turbidity and OD600 measurements displayed similar growth curves, while cfu quantification showed a continuous growth curve. We determined the cfu equivalents to McFarland and OD600 units.

Palavras-chave

McFarland; Mycobacterium tuberculosis; bacterial growth; colony forming units; optical density

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Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Idioma: En Revista: Braz J Microbiol Ano de publicação: 2013 Tipo de documento: Article

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