[Effect of osthole on bone metabolism in rat femoral tissues in vitro].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
; 35(5): 561-6, 2013 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-24183047
ABSTRACT
OBJECTIVE:
To investigate the effect of osthole on bone metabolism in rat femoral tissues in vitro.METHODS:
The rat femoral tissues were isolated in vitro. The optimal concentrations of ostehole (1×10(-5) mol/L) and estradiol (1×10(-8) mol/L) (the positive control) were selected by alkaline phosphatase activity (ALP). The ALP and calcium levels were detected by commmerical regents, and the expressions of osteoprotegerin, receptor activator of nuclear factor-κB ligand, runx-related gene 2, and bone morphogenetic protein-2 mRNA were determined by real-time reverse transcription-polymerase chain reaction.RESULT:
The osthole (1×10(-5) mol/L) significantly increased the activity of ALP, calcium level as well as the expressions of osteoprotegerin, receptor activator of nuclear factor-κB ligand, runx-related gene-2 and bone morphogenetic protein-2 mRNA in rat femoral tissues in vitro.CONCLUSION:
Osthole can improve calcium level and ALP activity and regulate the bone metabolism-related genes in rat femoral tissues.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cumarínicos
/
Fêmur
Limite:
Animals
Idioma:
Zh
Revista:
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
Ano de publicação:
2013
Tipo de documento:
Article