[Effect of fluoride on the expression of endoplasmic reticulum chaperone in ameloblast of rat incisor].
Shanghai Kou Qiang Yi Xue
; 22(5): 481-6, 2013 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-24233194
ABSTRACT
PURPOSE:
To investigate the effect of different concentrations of fluoride on the expression of endoplasmic reticulum chaperone, and to explore the mechanism of dental fluorosis in rat.METHODS:
Thirty Wistar rats were randomly divided into 3 groups. Immunohistochemistry was used to detect the expression of CRT, GRP78, XBP-1 and caspase-12 in rat incisors. Metamorph microscope images analysis system and SPSS 13.0 software package was used to analyze the data.RESULTS:
Typical features of dental fluorosis were found in the fluoride group. Results of immunohistochemistry showed that CRT (F=238.6, P<0.05), GRP78 (F=27.42, P<0.05), XBP-1 (F=139.7, P<0.05) and caspase-12 (F=43.91, P<0.05) were significantly different among the 3 groups.CONCLUSIONS:
Excessive fluoride can increase the secretion of CRT, GRP78, XBP-1 and caspase-12 suggest the ameloblasts and in status of endoplasmic reticulum stress and caspase-12 plays an important role during ameloblast apoptosis. Supported by National Natural Science Foundation of China (81072245) and Natural Science Foundation of Liaoning Province (20102278).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ameloblastos
/
Incisivo
Limite:
Animals
Idioma:
Zh
Revista:
Shanghai Kou Qiang Yi Xue
Ano de publicação:
2013
Tipo de documento:
Article