Comprehensive determination of the cyclic FEE peptide chemical stability in solution.
J Pharm Biomed Anal
; 89: 50-5, 2014 Feb.
Article
em En
| MEDLINE
| ID: mdl-24252725
ABSTRACT
The FEE cyclic hexapeptide (cFEE) is an investigational new drug added to the insemination medium in order to improve the in vitro fertilization rate. The pharmacological activity of small peptides is highly dependent on the conservation of the amino acid sequence and of the structural conformation of the active site. To enhance the scientific knowledge required for the clinical use of cFEE, a comprehensive determination of its chemical stability in solution was realized in accelerated conditions. Degradation products have been detected and identified by liquid chromatography/Qtrap(®) mass spectrometry. The main degradation products highlighted during the product shelf life were produced by hydrolysis and only certain sites were involved. In most cases, the cyclic conformation was lost and regarding the major degradation pathway, the sequence representing the active site was affected.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos Cíclicos
/
Soluções
Idioma:
En
Revista:
J Pharm Biomed Anal
Ano de publicação:
2014
Tipo de documento:
Article