Salivary lysosomal exoglycosidases profiles in patients with insulin-dependent and noninsulin-dependent diabetes mellitus.
Adv Clin Exp Med
; 22(5): 659-66, 2013.
Article
em En
| MEDLINE
| ID: mdl-24285450
ABSTRACT
BACKGROUND:
In this study we have investigated the effects of type I (insulin-dependent) and II (non-insulin dependent) diabetes mellitus on the specific activity and the output of salivary exoglycosidases N-acetyl-ß-hexosoaminidase (HEX), and its isoenzymes A and B (HEX A, HEX B), and ß-glucuronidase (GLU) in well controlled diabetic patients compared to healthy age-matched controls. MATERIAL ANDMETHODS:
In the saliva HEX, HEX A, HEX B and GLU were determined according to Marciniak et al. Protein was determined by the Lowry et al. method.RESULTS:
Our results show that in the case of type I diabetes, the significantly increased activity of salivary total HEX is mainly due to the significantly increased HEX A specific activity. Significantly increased HEX specific activity in DM II is an outcome of significantly increased HEX A as well as HEX B specific activities in comparison to the appropriate healthy control. Our results showed a significant increase in the specific activity of GLU in saliva of type II diabetes patients. The output of lysosomal exoglycosidases showed a similar significant increase compared to the healthy control, in both groups of diabetes mellitus patients.CONCLUSIONS:
This study has demonstrated that non-insulin dependent diabetes mellitus more strongly modify salivary glands glycoconjugates catabolism, which can be attributed to functional and morphological changes. A significant increase in the outputs of exoglycosidases in saliva of both type diabetes patients once more indicates that special attention should be paid to the oral health of these patients.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Diabetes Mellitus Tipo 1
/
Diabetes Mellitus Tipo 2
/
Glicosídeo Hidrolases
Limite:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Adv Clin Exp Med
Ano de publicação:
2013
Tipo de documento:
Article