Genetic variability of Dactylopius opuntiae (Hemiptera, Dactylopiidae) on forage cactus in northeast Brazil.
Genet Mol Res
; 12(4): 5236-46, 2013 Oct 30.
Article
em En
| MEDLINE
| ID: mdl-24301784
ABSTRACT
The carmine cochineal Dactylopius opuntiae is a key pest in productive fields of forage cactus in Pernambuco, Brazil. Species identification by means of molecular markers assists in understanding the genetic profile, underpins morphological characterization, and supports the monitoring of populations in integrated management programs designed to control this pest. We evaluated the genetic variability of natural populations of D. opuntiae. Genetic variability was analyzed with ISSR and RAPD primers in 24 populations from 12 municipalities of Pernambuco State in Brazil. Morphological characterization confirmed that D. opuntiae was the only cochineal species present in all samples. Nine ISSR primers and six RAPD produced a total of 62 and 58 polymorphic fragments, respectively. Both types of markers showed an average genetic similarity of 80% regardless of the geographic origin of samples. The low genetic variability demonstrates a high degree of relatedness among these D. opuntiae populations.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Variação Genética
/
Cactaceae
/
Hemípteros
Tipo de estudo:
Prognostic_studies
Limite:
Animals
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Genet Mol Res
Ano de publicação:
2013
Tipo de documento:
Article