A new transfectant panel cell line-based MoAb-independent antigen capture assay system for detection of CD36 antibody.
Vox Sang
; 106(4): 368-71, 2014 May.
Article
em En
| MEDLINE
| ID: mdl-24387678
ABSTRACT
BACKGROUND AND OBJECTIVES:
CD36 antibody (Ab) causes several disorders neonatal alloimmune thrombocytopenia, platelet transfusion refractoriness and non-haemolytic transfusion reactions. However, there is no gold-standard test for CD36 Ab. MATERIALS ANDMETHODS:
We developed a transfectant panel cell line-based MoAb-independent antigen capture assay system for detection of CD36 Ab and compared it with the monoclonal antibody-specific immobilization of platelet antigens (MAIPA) system in terms of sensitivity and specificity.RESULTS:
Our new system was characterized by (1) gene-transfected cell lines, but not panel platelets; (2) not being hampered by HLA Abs; and (3) no need to use CD36 MoAbs to ensure the antigen specificity of this detection system. In addition, it showed a much better receiver operating characteristic curve than the MAIPA system.CONCLUSIONS:
The present results indicate that our new system permits highly sensitive and specific detection of CD36 Ab.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Autoanticorpos
/
Antígenos CD36
/
Anticorpos Monoclonais
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Vox Sang
Ano de publicação:
2014
Tipo de documento:
Article