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Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus.
Mok, Chee-Keng; Chang, Shih-Cheng; Chen, Guang-Wu; Lo, Yu-Lun; Chen, Shu-Jen; Wu, Ho-Sheng; Liu, Ming-Tsan; Chang, Feng-Yee; Lin, Tzou-Yien; Shih, Shin-Ru.
Afiliação
  • Mok CK; Research Center for Emerging Viral Infections, Chang Gung University and Clinical Virology Laboratory, Chang Gung Memorial Hospital, Taiwan Centers for Disease Control Collaborating Laboratory of Virology, Taoyuan, Taiwan, ROC; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gu
  • Chang SC; Research Center for Emerging Viral Infections, Chang Gung University and Clinical Virology Laboratory, Chang Gung Memorial Hospital, Taiwan Centers for Disease Control Collaborating Laboratory of Virology, Taoyuan, Taiwan, ROC.
  • Chen GW; Research Center for Emerging Viral Infections, Chang Gung University and Clinical Virology Laboratory, Chang Gung Memorial Hospital, Taiwan Centers for Disease Control Collaborating Laboratory of Virology, Taoyuan, Taiwan, ROC; Department of Computer Science and Information Engineering, College of E
  • Lo YL; Research Center for Emerging Viral Infections, Chang Gung University and Clinical Virology Laboratory, Chang Gung Memorial Hospital, Taiwan Centers for Disease Control Collaborating Laboratory of Virology, Taoyuan, Taiwan, ROC.
  • Chen SJ; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan, ROC; Molecular Medicine Research Center, Chang Gung University, Taoyuan, Taiwan, ROC.
  • Wu HS; Research and Diagnostics Center, Centers for Disease Control, Taipei, Taiwan, ROC.
  • Liu MT; Research and Diagnostics Center, Centers for Disease Control, Taipei, Taiwan, ROC.
  • Chang FY; Research and Diagnostics Center, Centers for Disease Control, Taipei, Taiwan, ROC.
  • Lin TY; Ministry of Health and Welfare, Executive Yuan, Taipei, Taiwan, ROC.
  • Shih SR; Research Center for Emerging Viral Infections, Chang Gung University and Clinical Virology Laboratory, Chang Gung Memorial Hospital, Taiwan Centers for Disease Control Collaborating Laboratory of Virology, Taoyuan, Taiwan, ROC; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gu
J Microbiol Immunol Infect ; 48(4): 465-9, 2015 Aug.
Article em En | MEDLINE | ID: mdl-24388586
ABSTRACT
Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan's first imported human case and found R and K in quasispecies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antivirais / Proteínas Virais / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Farmacorresistência Viral / Oseltamivir / Subtipo H7N9 do Vírus da Influenza A / Neuraminidase Limite: Humans País/Região como assunto: Asia Idioma: En Revista: J Microbiol Immunol Infect Ano de publicação: 2015 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antivirais / Proteínas Virais / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Farmacorresistência Viral / Oseltamivir / Subtipo H7N9 do Vírus da Influenza A / Neuraminidase Limite: Humans País/Região como assunto: Asia Idioma: En Revista: J Microbiol Immunol Infect Ano de publicação: 2015 Tipo de documento: Article