The EHV-1 UL4 protein that tempers viral gene expression interacts with cellular transcription factors.
Virology
; 449: 25-34, 2014 Jan 20.
Article
em En
| MEDLINE
| ID: mdl-24418534
The UL4 gene is conserved within the genome of defective interfering particles of equine herpesvirus type 1 (EHV-1) that mediate persistent infection. Here, we show that the UL4 protein inhibits EHV-1 reporter gene expression by decreasing the level of transcribed mRNA. The UL4 protein did not bind any gene class of EHV-1 promoters in electromobility or chromatin immunoprecipitation assays, but directly interacted with the TATA box-binding protein (TBP) and the carboxy-terminal domain of RNA polymerase II both in vitro (GST-pulldown assays) and in infected cells (coimmunoprecipitation analyses). Microarray analyses of the expression of the 78 EHV-1 genes revealed that viral late genes important for virion assembly displayed enhanced expression in cells infected with UL4-null virus as compared to wild-type or UL4-restored EHV-1. Quantitative PCR analyses showed that viral DNA replication was not retarded in cells infected with the UL4-null virus as compared to wild-type EHV-1.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
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RNA Polimerase II
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Regulação Viral da Expressão Gênica
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Herpesvirus Equídeo 1
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Infecções por Herpesviridae
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Proteína de Ligação a TATA-Box
Limite:
Animals
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Humans
Idioma:
En
Revista:
Virology
Ano de publicação:
2014
Tipo de documento:
Article