The raising and characterisation of antibodies to salicylate.
Ann Clin Biochem
; 24 ( Pt 4): 374-84, 1987 Jul.
Article
em En
| MEDLINE
| ID: mdl-2444149
ABSTRACT
Polarisation fluorescence was used to assess the antibody response in sheep immunised with 4-aminosalicylic acid coupled to keyhole limpet haemocyanin and to develop a polarisation fluoroimmunoassay for subsequent clinical use. The fluorescent label was prepared by coupling 4-aminosalicylic acid to fluorescein isothiocyanate. All immunised sheep produced antibodies and a simple assay was developed using salicylic acid as the standard. The simple fluorescence polarisation approach enabled the rapid and direct study of the immuno-reactivity of a large number of pure chemical analogues of salicylic acid. Each was assessed for its ability to compete with the fluorophore-labelled analyte for antibody-binding sites, relative to salicylate, thereby enabling investigation of the steric and chemical characteristics important in the recognition and binding of a hapten to antibodies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácido Aminossalicílico
/
Ácidos Aminossalicílicos
/
Anticorpos
Limite:
Animals
Idioma:
En
Revista:
Ann Clin Biochem
Ano de publicação:
1987
Tipo de documento:
Article