In vitro studies on the interaction between human serum albumin and fosfomycin disodium salt, an antibiotic drug by multi-spectroscopic and molecular docking methods.
Mol Biol Rep
; 41(4): 2377-87, 2014.
Article
em En
| MEDLINE
| ID: mdl-24443227
ABSTRACT
The interaction between the human serum albumin (HSA) and drug, fosfomycin disodium salt (FOS) has been studied by different spectroscopic techniques. The experimental results showed a static quenching mechanism in the interaction of FOS with HSA. The number of binding sites, n and observed binding constant K a were measured by fluorescence quenching method. The thermodynamic parameters ΔG°, ΔH° and ΔS° were calculated according to van't Hoff equation. The calculated distance r between FOS and the protein is evaluated according to the theory of Förster energy transfer. A change in the secondary structure of the protein was evident from the circular dichroism measurements, synchronous fluorescence and three-dimensional fluorescence spectra.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Análise Espectral
/
Albumina Sérica
/
Simulação de Acoplamento Molecular
/
Fosfomicina
/
Antibacterianos
Limite:
Humans
Idioma:
En
Revista:
Mol Biol Rep
Ano de publicação:
2014
Tipo de documento:
Article