Case study 3. Application of basic enzyme kinetics to metabolism studies: real-life examples.
Methods Mol Biol
; 1113: 441-60, 2014.
Article
em En
| MEDLINE
| ID: mdl-24523124
ABSTRACT
An appreciation of the principles of enzyme kinetics can be applied in a number of drug metabolism applications. The concept for this chapter arose from a simple discussion on selecting appropriate time points to most efficiently assess metabolite profiles in a human Phase 1a clinical study (Subheading 4). By considering enzyme kinetics, a logical approach to the issue was derived. The dialog was an important learning opportunity for the participants in the discussion, and we have endeavored to capture this experience with other questions related to determination of K m and V max parameters, a consideration of the value of hepatocytes versus liver microsomes and enzyme inhibition parameters.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Preparações Farmacêuticas
/
Enzimas
/
Ensaios Enzimáticos
Tipo de estudo:
Guideline
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2014
Tipo de documento:
Article