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Biodentine and mineral trioxide aggregate induce similar cellular responses in a fibroblast cell line.
Corral Nuñez, Camila M; Bosomworth, Helen J; Field, Claire; Whitworth, John M; Valentine, Ruth A.
Afiliação
  • Corral Nuñez CM; Centre for Oral Health Research, School of Dental Sciences, Newcastle University, Newcastle upon Tyne, UK.
  • Bosomworth HJ; Centre for Oral Health Research, School of Dental Sciences, Newcastle University, Newcastle upon Tyne, UK.
  • Field C; Centre for Oral Health Research, School of Dental Sciences, Newcastle University, Newcastle upon Tyne, UK.
  • Whitworth JM; Centre for Oral Health Research, School of Dental Sciences, Newcastle University, Newcastle upon Tyne, UK.
  • Valentine RA; Centre for Oral Health Research, School of Dental Sciences, Newcastle University, Newcastle upon Tyne, UK. Electronic address: ruth.valentine@newcastle.ac.uk.
J Endod ; 40(3): 406-11, 2014 Mar.
Article em En | MEDLINE | ID: mdl-24565661
ABSTRACT

INTRODUCTION:

The aim of this study was to assess the cell viability and messenger RNA expression of interleukin (IL)-1α and IL-6 in 3T3 fibroblast cells when in direct contact with Biodentine (Septodont, Saint Maur de Fossés, France) and mineral trioxide aggregate (MTA).

METHODS:

Biodentine and MTA were coated onto coverslips and allowed to set. An uncoated coverslip and one coated with GC Fuji IX (GC Corporation, Tokyo, Japan) were used as controls. Coverslips were cultured with 3T3 fibroblast cells. Cell viability was assessed quantitatively using AlamarBlue dye (Serotec, Oxford, UK) after 3, 6, 24, and 72 hours. Morphologic cell changes of 3T3 cells in contact with BD and MTA were observed by scanning electron microscopy, and cytokine expression was assessed at the messenger RNA level by semiquantitative reverse-transcription polymerase chain reaction after 3 and 24 hours of direct contact with the materials.

RESULTS:

Cells in contact with Biodentine and MTA showed similar viability to untreated control cells at all time points, with the exception of 6 hours when viability was decreased with both treatments. Examination by scanning electron microscopy revealed cells adhering to most of the Biodentine surface after 24 hours. However, for MTA samples, significantly fewer cells were observed. The messenger RNA expression of IL-1α and IL-6 by cells in contact with Biodentine was similar to cells in contact with MTA.

CONCLUSIONS:

Biodentine and MTA showed similar cytotoxicity and induced a similar pattern of cytokine expression.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óxidos / Materiais Restauradores do Canal Radicular / Silicatos / Compostos de Cálcio / Compostos de Alumínio / Agentes de Capeamento da Polpa Dentária e Pulpectomia / Fibroblastos Limite: Animals Idioma: En Revista: J Endod Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óxidos / Materiais Restauradores do Canal Radicular / Silicatos / Compostos de Cálcio / Compostos de Alumínio / Agentes de Capeamento da Polpa Dentária e Pulpectomia / Fibroblastos Limite: Animals Idioma: En Revista: J Endod Ano de publicação: 2014 Tipo de documento: Article