High Level Expression and Purification of Atl, the Major Autolytic Protein of Staphylococcus aureus.
Int J Microbiol
; 2014: 615965, 2014.
Article
em En
| MEDLINE
| ID: mdl-24669224
ABSTRACT
Staphylococcus aureus is a major human and animal pathogen. Autolysins regulate the growth, turnover, cell lysis, biofilm formation, and the pathogenicity of S. aureus. Atl is the major autolysin in S. aureus. The biochemical and structural studies of staphylococcal Atl have been limited due to difficulty in cloning, high level overexpression, and purification of this protein. This study describes successful cloning, high level over-expression, and purification of two forms of fully functional Atl proteins. These pure proteins can be used to study the functional and structural properties of this important protein.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Int J Microbiol
Ano de publicação:
2014
Tipo de documento:
Article