Transient state imaging of live cells using single plane illumination and arbitrary duty cycle excitation pulse trains.
J Biophotonics
; 8(5): 392-400, 2015 May.
Article
em En
| MEDLINE
| ID: mdl-24706633
ABSTRACT
We demonstrate the applicability of Single Plane Illumination Microscopy to Transient State Imaging (TRAST), offering sensitive microenvironmental information together with optical sectioning and reduced overall excitation light exposure of the specimen. The concept is verified by showing that transition rates can be determined accurately for free dye in solution and that fluorophore transition rates can be resolved pixel-wise in live cells. Furthermore, we derive a new theoretical framework for analyzing TRAST data acquired with arbitrary duty cycle pulse trains. By this analysis it is possible to reduce the overall measurement time and thereby enhance the frame rates in TRAST imaging.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microscopia de Fluorescência
Limite:
Humans
Idioma:
En
Revista:
J Biophotonics
Ano de publicação:
2015
Tipo de documento:
Article