Lack of group X secreted phospholipase A2 increases survival following pandemic H1N1 influenza infection.
Virology
; 454-455: 78-92, 2014 Apr.
Article
em En
| MEDLINE
| ID: mdl-24725934
ABSTRACT
The role of Group X secreted phospholipase A2 (GX-sPLA2) during influenza infection has not been previously investigated. We examined the role of GX-sPLA2 during H1N1 pandemic influenza infection in a GX-sPLA2 gene targeted mouse (GX(-/-)) model and found that survival after infection was significantly greater in GX(-/-) mice than in GX(+/+) mice. Downstream products of GX-sPLA2 activity, PGD2, PGE2, LTB4, cysteinyl leukotrienes and Lipoxin A4 were significantly lower in GX(-/-) mice BAL fluid. Lung microarray analysis identified an earlier and more robust induction of T and B cell associated genes in GX(-/-) mice. Based on the central role of sPLA2 enzymes as key initiators of inflammatory processes, we propose that activation of GX-sPLA2 during H1N1pdm infection is an early step of pulmonary inflammation and its inhibition increases adaptive immunity and improves survival. Our findings suggest that GX-sPLA2 may be a potential therapeutic target during influenza.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por Orthomyxoviridae
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Vírus da Influenza A Subtipo H1N1
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Fosfolipases A2 do Grupo X
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Virology
Ano de publicação:
2014
Tipo de documento:
Article