Enzymatic formation of gold nanoparticles by submerged culture of the basidiomycete Lentinus edodes.
J Biotechnol
; 182-183: 37-45, 2014 Jul 20.
Article
em En
| MEDLINE
| ID: mdl-24800960
ABSTRACT
We report for the first time that the medicinal basidiomycete Lentinus edodes can reduce Au(III) from chloroauric acid (HAuCl4) to elemental Au [Au(0)], forming nanoparticles. Several methods, including transmission electron microscopy, electron energy loss spectroscopy, X-ray fluorescence, and dynamic light scattering, were used to show that when the fungus was grown submerged, colloidal gold accumulated on the surface of and inside the mycelial hyphae as electron-dense particles mostly spherical in shape, with sizes ranging from 5 to 50nm. Homogeneous proteins (the fungal enzymes laccase, tyrosinase, and Mn-peroxidase) were found for the first time to be involved in the reduction of Au(III) to Au(0) from HAuCl4. A possible mechanism forming Au nanoparticles is discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Cultura de Células
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Cogumelos Shiitake
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Nanopartículas Metálicas
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Ouro
Idioma:
En
Revista:
J Biotechnol
Ano de publicação:
2014
Tipo de documento:
Article