Continuous fever-range heat stress induces thermotolerance in odontoblast-lineage cells.
Arch Oral Biol
; 59(7): 741-8, 2014 Jul.
Article
em En
| MEDLINE
| ID: mdl-24814171
ABSTRACT
OBJECTIVE:
Heat shock during restorative procedures can trigger damage to the pulpodentin complex. While severe heat shock has toxic effects, fever-range heat stress exerts beneficial effects on several cells and tissues. In this study, we examined whether continuous fever-range heat stress (CFHS) has beneficial effects on thermotolerance in the rat clonal dental pulp cell line with odontoblastic properties, KN-3.METHODS:
KN-3 cells were cultured at 41°C for various periods, and the expression level of several proteins was assessed by Western blot analysis. After pre-heat-treatment at 41°C for various periods, KN-3 cells were exposed to lethal severe heat shock (LSHS) at 49°C for 10min, and cell viability was examined using the MTS assay. Additionally, the expression level of odontoblast differentiation makers in surviving cells was examined by Western blot analysis.RESULTS:
CFHS increased the expression levels of several heat shock proteins (HSPs) in KN-3 cells, and induced transient cell cycle arrest. KN-3 cells, not pre-heated or exposed to CFHS for 1 or 3h, died after exposure to LSHS. In contrast, KN-3 cells exposed to CFHS for 12h were transiently lower on day 1, but increased on day 3 after LSHS. The surviving cells expressed odontoblast differentiation markers, dentine sialoprotein and dentine matrix protein-1. These results suggest that CFHS for 12h improves tolerance to LSHS by inducing HSPs expression and cell cycle arrest in KN-3 cells.CONCLUSIONS:
The appropriate pretreatment with continuous fever-range heat stress can provide protection against lethal heat shock in KN-3 cells.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
6_ODS3_enfermedades_notrasmisibles
Base de dados:
MEDLINE
Assunto principal:
Resposta ao Choque Térmico
/
Proteínas de Choque Térmico
/
Odontoblastos
Limite:
Animals
Idioma:
En
Revista:
Arch Oral Biol
Ano de publicação:
2014
Tipo de documento:
Article