Genome-wide analysis reveals novel and discrete functions for tubulin carboxy-terminal tails.
Curr Biol
; 24(12): 1295-1303, 2014 Jun 16.
Article
em En
| MEDLINE
| ID: mdl-24835459
BACKGROUND: Microtubules (MTs) support diverse transport and force generation processes in cells. Both α- and ß-tubulin proteins possess carboxy-terminal tail regions (CTTs) that are negatively charged, intrinsically disordered, and project from the MT surface where they interact with motors and other proteins. Although CTTs are presumed to play important roles in MT networks, these roles have not been determined in vivo. RESULTS: We examined the function of CTTs in vivo by using a systematic collection of mutants in budding yeast. We find that CTTs are not essential; however, loss of either α- or ß-CTT sensitizes cells to MT-destabilizing drugs. ß-CTT, but not α-CTT, regulates MT dynamics by increasing frequencies of catastrophe and rescue events. In addition, ß-CTT is critical for the assembly of the mitotic spindle and its elongation during anaphase. We use genome-wide genetic interaction screens to identify roles for α- and ß-CTTs, including a specific role for ß-CTT in supporting kinesin-5/Cin8. Our genetic screens also identified novel interactions with pathways not related to canonical MT functions. CONCLUSIONS: We conclude that α- and ß-CTTs play important and largely discrete roles in MT networks. ß-CTT promotes MT dynamics. ß-CTT also regulates force generation in the mitotic spindle by supporting kinesin-5/Cin8 and dampening dynein. Our genetic screens identify links between α- and ß-CTT and additional cellular pathways and suggest novel functions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Tubulina (Proteína)
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Proteínas de Saccharomyces cerevisiae
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Microtúbulos
Idioma:
En
Revista:
Curr Biol
Ano de publicação:
2014
Tipo de documento:
Article