Real-time luminescence microspectroscopy monitoring of singlet oxygen in individual cells.
Photochem Photobiol Sci
; 13(8): 1203-12, 2014 Aug.
Article
em En
| MEDLINE
| ID: mdl-24954013
ABSTRACT
A new setup for direct microspectroscopic monitoring of singlet oxygen ((1)O2) has been developed in our laboratory using a novel near-infrared sensitive InGaAs 2D-array detector. An imaging spectrograph has been inserted in front of the 2D-array detector, which allows us to acquire spectral images where one dimension is spatial and the other is spectral. The work presents a detailed examination of sensitivity and noise characteristics of the setup and its ability to detect (1)O2. The (1)O2 phosphorescence-based images and near-infrared luminescence spectral images recorded from single TMPyP-containing fibroblast cells reflecting spectral changes during irradiation are demonstrated. The introduction of spectral images addresses the issue of a potential spectral overlap of (1)O2 phosphorescence with near-infrared-extended luminescence of the photosensitizer and provides a powerful tool for distinguishing and separating them, which can be applied to any photosensitizer manifesting near-infrared luminescence.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microespectrofotometria
/
Oxigênio Singlete
/
Análise de Célula Única
Limite:
Animals
Idioma:
En
Revista:
Photochem Photobiol Sci
Ano de publicação:
2014
Tipo de documento:
Article